Fig 2.
Expression of fluorescent reporters during planktonic growth. S. aureus UAMS-1 (WT), the cidR mutant (ΔcidR) strain, and the lytSR mutant (ΔlytSR) strain containing the cid::gfp, lrg::gfp, and ldh::gfp transcriptional reporter plasmids were grown to exponential phase in the presence or absence of 35 mM glucose and under aerobic and hypoxic conditions as indicated. GFP-positive cells were visualized by CLSM at ×630 magnification as described in Materials and Methods. The scale bar represents 5 μm.