Figure 4. GFP expression in the livers of TG mice after injury.
GFAP-Cre/GFP mice (n = 15) were fed MCDE-diets and survivors were sacrificed after 1 week (n = 4) or 3 weeks (n = 4). Other mice that survived 3 weeks of MCDE treatment (n = 4) were sacrificed after being switched back to normal diets for 3 weeks. GFP expression was assessed by immunohistochemistry. Representative results in mice that were sacrificed at the end of 1 week MCDE diet treatment (A, B), 3 weeks MCDE treatment (C, D), or 3 weeks after stopping a 3 week course of MDCE diets (E, F). Numbers of GFP-positive cells visualized at X40 were counted in different parts of the liver lobule (i.e., near central veins (CV) and portal tracts (PT)) (G). * < p 0.05, ** p < 0.005 (A, C, E original magnification X10; B, D, F X63). Arrows and arrowhead indicate portal triads and central veins, respectively.