Figure 4. Genetic disruption of the Miz1 POZ domain upregulates Cebpd transcription in vivo and in vitro.
(a) Miz1(POZ)fl/fl mice were intratracheally transduced with Ad-null or Ad-Cre for 30 d and then intratracheally treated with PBS or LPS for 8 h. Protein and mRNA abundance of C/EBP isoforms in the lungs were determined. (b,c) Analysis of C/EBP-δ protein and mRNA expression in ATII cells that were isolated from Miz1(POZ)fl/fl mice, infected with Ad-null or Ad-Cre, and then treated with TNF (b) or LPS (c) for 4 h. (d,e) Protein or mRNA levels of C/EBPs in MLE12–Miz1(WT) and MLE-12–Miz1(ΔPOZ) cells treated with TNF (d) or LPS (e) for 4 h. Results are representative of at least three independent experiments (a–e). (f,g) Analysis of mRNA levels of C/EBP-δ (f) and IL-6 (g) in MLE12–Miz1(WT) and MLE-12–Miz1(ΔPOZ) cells treated with LPS for different times as indicated. Data are representative of three independent experiments (means ± s.e.m.).