Figure 5. Treatment with Hydrogen Peroxide and Beta-Mercaptoethanol do not affect TPIsugarkill protein stability.
Western blots were conducted on fly extracts from homozygous TPIsugarkill animals 48 hours after treatment with the indicated compounds at 25C. A) The average relative amount of TPI in each extract type is denoted. The levels of protein were compared to the vehical only control (-). Data were not found to be significantly different between experimental and control samples (Student’s t-test. N = 6). B) A representative western blot demonstrating the similar levels of TPIsugarkill protein in the flies treated with hydrogen peroxide and beta-mercaptoethanol. Beta-tubulin was included as a loading control in the experiment.