Figure 7.
Pathogenic role of C3.
A. C3 protein levels measured by ELISA in the serum from Stat3WT/WT (white bars, n = 26), non-infiltrated (grey bars, n = 26) and infiltrated (black bars, n = 28) Stat3C/C mice. Data are shown as mean ± SEM. *p = 0.034; ***p < 0.0001.
B. C3 mRNA levels measured by Taqman RT-PCR on liver RNA from the same mice as in (A). Data are shown as mean ± SEM. *p = 0.013 (Stat3WT/WT vs non-infiltrated Stat3C/C); **p = 0.0086 (Stat3WT/WT vs infiltrated Stat3C/C).
C. Survival curves of mice after in vivo complement depletion. CVF-treated Stat3WT/WT (black line, n = 8), non-infiltrated (orange line, n = 7) and infiltrated (red line, n = 12) Stat3C/C mice were analysed. ***p < 0.0001.
D. IF analysis of C3 localization in the hearts of Stat3C/C and Stat3WT/WT mice at the indicated ages in weeks. Green: CD4; Red: C3. Pictures are representative of six mice per genotype. Scale bar: 50 µm.
E. Immunofluorescence analysis of IgG deposition (in red) in the same samples from (D). Scale bar: 50 µm.