Figure 3. Comparison of soluble and immobilized growth factors in a 3-day cultivation of H5V endothelial cells.
Soluble VEGF (50ng/mL) and Ang1 (50ng/mL) were applied to cell-seeded collagen scaffolds treated with PBS alone (S-(VEGF+Ang1)), or scaffolds treated with EDC crosslinker in PBS (S-(VEGF+Ang1)+EDC). These scaffolds were compared to scaffolds with co-immobilized VEGF and Ang1 (VEGF+Ang1). 50,000 cells were seeded on the freshly made scaffolds. (A) XTT assay indicating final cell numbers in collagen scaffolds. (B) Lactate production rate. (C) Glucose consumption rate. * denotes statistically significant difference (P < 0.05; one-way ANOVA with post-hoc Tukey test). Figure reproduced from [14] with permission. Copyright Elsevier.