Fig. 7. Abnormal phenotype of forestomach of Sox2EGFP/LP mutants.

Sections of the wild type (A-E) and mutant (F-J) P0 anterior stomach were stained with anti-Keratin14 (A,F), anti-p63 (B,G), anti-involucrin (C,H), Alcian blue (D,I) and Muc5AC (E,J). Note that Keratin14 expression is reduced or absent (arrowheads) in the mutant forestomach (F). Likewise, anti-p63 immunostaining shows regions of reduced or absent staining in the mutant forestomach (G). Ectopic Alcian blue-positive (I) and Muc5AC-positive (J, arrowheads) cells are present in the mutant forestomach. Scale bars: 50 μm.