Fig. 5.
Imaging of neuron–single-RBC coupling in mouse visual cortex. (A) Schematic of the experimental setup. (B) Transient responses to a single visual stimulation. The sO2,
, vf, and rO2 have statistically significant changes according to two-way ANOVA tests (n = 4). Error bars denote SEMs. *P < 0.05, **P < 0.01, ***P < 0.001. (C) Representative MAP images without continuous visual stimulation. (D) Representative MAP images with 1-Hz continuous optical flashing stimulations on the left mouse eye. Scale bars in C and D: x = 10 µm, t = 10 s. (E) Relative changes of single-RBC functional parameters under continuous visual stimulation. Values are normalized to mean values of control images. The <sO2>,
, vf, and rO2 are significantly different from their control values according to two-way ANOVA tests (n = 3). Error bars are SEMs. *P < 0.05. (F–I) CDFs of <sO2>,
, vf, and rO2 under control (ctrl) and stimulation (stim). rO2 was normalized to the mean value of the control experiment.
