Fig 4.
Equilibrium ultracentrifugation of JFH-1 particles in isopycnic iodixanol gradients. Filtered supernatant fluids collected from JFH-1 RNA- and pre- or anti-miRNA-transfected Huh-7.5 cell cultures were concentrated and used to collect fractions (500 μl each). Black circles indicate the gradient densities of the fractions, white circles indicate the HCV RNA titers, and bars indicate HCV infectivity levels. Panels: A, cells overexpressing pre-miR-control; B, cells overexpressing pre-miR-27a; C, cells overexpressing anti-miR-control; D, cells overexpressing anti-miR-27a. Experiments were repeated twice.