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Spectra of purified E101A and WT CcO with and without CHOBIMALT. Panel A: Soret region; panel B: α region. Spectra were normalized to the fully reduced α peak height at 607nm – 630nm. Spectra of fully oxidized and fully reduced purified CcO are shown in black. Steady-state spectra in 100mM CHES pH 9.5 with 0.01% DDM were recorded 3 minutes after adding 1 mM ascorbate and 200 μM TMPD: WT only (green), WT with CHOBIMALT (blue), E101A only (magenta), E101A with CHOBIMALT (red).
