Skip to main content
. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Nat Struct Mol Biol. 2013 Mar 24;20(4):426–432. doi: 10.1038/nsmb.2529

Figure 4. Actin subdomain 2 is crucial for INO80 interaction with chromatin.

Figure 4

(a,b) Western blot analysis of, (a) purified INO80-chromatin complex in presence and absence of MNase after complete subtilisin digestion, probed with polyclonal anti-β-actin antibodies, (b) subtilisin accessibility to actin subdomain 2 in purified INO80 complex without its substrate chromatin, in presence or absence of 147 Nuc or 207 Nuc. (c) SDS-PAGE and silver staining for INO80 complex (lane I), and INO80: DNase I complex (lane II). (d) Graph showing relative DNA or nucleosome (359 bp INO1) dependent ATPase activity of INO80: DNase I compared to INO80 complex using equimolar amount of both the complexes and data presented is the mean of five independent experiments ± S.D.