Figure 7.
Oct4/Sox2 and Oct4/Sox17 complexes regulate specific genes in mouse embryos at the blastocyst stage. (A) Sox17, Sox2, Oct4, Nanog and Gata4 expression levels distribution across each cell of a 64-cell stage mouse embryo obtained by single-cell gene expression analysis (Guo et al, 2010). A background of Ct=28 was used to obtain an absolute expression level. (B) Immunostainings of Oct4 and Sox17 in mouse embryos at mid and late blastocyst stage. Scale bar is 20 μm. (C) Histogram plots representing E3.75 blastocyst versus ICM expression level ratios for genes harbouring a compressed or a canonical motif in their enhancer region. Expression levels were determined by RNA-seq in RPKM (reads per kilobase per million mapped reads). (D) Histogram plot of the raw expression levels of each genes in ES and PrE cells with a compressed or a canonical motif and an E3.75 blastocyst/ICM lower than 1.