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Both fragments of split T7 RNAP are required for activity. (A) Plasmids containing just the N-terminal fragment, just the C-terminal fragment, or both were cotransformed with a reporter plasmid. (B) GFP fluorescence normalized to OD after 10 h incubation in either 0 or 10 mM arabinose for each version described in (A). Cells containing either half of split T7 RNAP show no GFP expression. When both halves of split T7 RNAP are present, however, high levels of GFP were found.
