Fig. 5.
RABV-mediated tracing of local presynaptic partners of adult-generated neurons in the OB. (A) Scheme of sequential virus delivery in two different injection paradigms (1 and 2). Injection of retrovirus into the SEZ, followed by RABV infection of migrating neuroblasts in the RMS (3 and 4). Injection of retrovirus into the SEZ, followed by RABV infection in the OB. (B) Double-transduced newborn granule cells at different stages of maturation obtained following injection schemes 1, 2, and 3. (Scale bar, 30 μm.) (C) Overview of RABV-labeled neurons in the OB following injection scheme 2. (Scale bar, 200 μm.) (D) Example of a double-transduced granule cell and RABV-traced superficial short axon cells (SACs) following RABV injection in the RMS. (Insets) Enlarged images of single and merged channels. (Scale bars, 50 μm.) (E) Example of a RABV-traced Blanes cell in the GCL. Arrowheads point to the emerging axon. (Scale bar, 50 μm.) (F) Example of a RABV-traced superficial SAC in the EPL. (Scale bar, 50 μm.) (G) Overview of double-transduced (yellow arrowheads) and RABV-traced local neurons following RABV injection into the OB. A 3× digital zoom of the boxed area shows double-transduced and RABV-only transduced neurons (white arrowheads). (Scale bars, 50 μm.) (H) RABV-traced deep SAC following RABV injection in the OB. Note the double-transduced cell (yellow arrowhead; Insets show single and merged channels); red arrowheads point to the ascending axon of the SAC. Boxed area (i) shows the cell body, with the emerging axon indicated by the arrowheads; area (ii) shows part of the axonal arborisation in the EPL. (Scale bars, 20 μm.)