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. 2013 Apr 1;140(7):1433–1444. doi: 10.1242/dev.087551

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© 2013. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 5. — Stem cell markers are not independently regulated. (A,B) QPCR expression data for zebrafish delta (A) and LRIG1 (B) in keratinocytes retrovirally infected with delta alone (D) or together with LRIG1 (DL); control cells were infected with empty vector (EV). (C,D) Immunostaining for zebrafish Delta (C, green) and FLAG (D, green) with DAPI counterstain (blue). (E-J) QPCR expression data for six genes identified as upregulated in D+ cells. n=4 experiments. ^*P<0.05; ^**P<0.01. (K) Quantitation of percentage spread cells after adhesion to type I collagen for 30 minutes. n=3 experiments. ^*P=0.0418 for DL; n.s.: not significant. (L) Immunostaining for EGFR (green) and CAV1 (red) with DAPI nuclear counterstain (blue). Cells were serum starved (0 minute) or treated with EGF for 20 minutes. (M) Quantitation of vesicles in L. n=3 experiments. Values above columns indicate fold-change compared with empty vector (EV) control. ^**P=0.00229 for D, ^**P=0.00828 for DL. Data are mean ± s.e.m. Scale bars: 50 μm.