FIGURE 2.
A, SirT1 and PGC-1α protein expression in vehicle and RSV-treated C2C12 myotubes, with or without stimulation using the 4-day protocol. B and C, SirT1 and PGC-1α protein expression data shown graphically. D, immunofluorescence of SirT1 (red) and PGC-1α (green) with DAPI (blue)-stained nuclei. Images examine SirT1 and PGC-1α nuclear localization in C212 myotubes treated with vehicle or RSV, with and without CCA using the 4-day protocol (n = 9, arbitrary scanner units (A.U.) corrected for loading using α-tubulin; *, p < 0.05, overall effect of stimulation versus control (Con); ‡, p < 0.05, overall effect of RSV versus control).