Table 3.
Photophysical parameters of RCaMPs, R-GECO1, and parent fluorescent proteins.
| One-photon excitation | Two-photon excitation | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| λabs (λem), nm | εpeak (mM−1cm−1) | Quantum yield | Brightness, % (relative to EGFP) | ΔF/F 570ex, all em. | ΔF/F 1070ex, all em. | λabs (nm) | Peak brightness, 1070 nm (kcpsm) | |||||||||
| +Ca2+ | −Ca2+ | +Ca2+ | −Ca2+ | +Ca2+ | −Ca2+ | +Ca2+ | −Ca2+ | +Ca2+ | −Ca2+ | +Ca2+ | ||||||
| mRuby | 558(590) | 558(590) | 94.6 | 0.41 | 113 | 1060 | 4.0 | |||||||||
| RCaMP1a | 576.5(595) | 575.5(594) | 56.7 | 34.8 | 0.45 | 0.12 | 75 | 12 | 4.1 | 4.3 | 1070 | 1070 | 5.9 | |||
| RCaMP1c | 576.5(595) | 576.5(597) | 64.8 | 39.7 | 0.48 | 0.10 | 91 | 11.6 | 5.9 | 7.1 | 1070 | 1070 | 7.3 | |||
| RCaMP1d | 572(592.5) | 575(597.5) | 57.8 | 25.4 | 0.52 | 0.13 | 88 | 9.6 | 8.7 | 11.1 | 1070 | 1070 | 7.7 | |||
| RCaMP1f | 572(591.5) | 574(597) | 58.9 | 17.4 | 0.48 | 0.11 | 83 | 5.6 | 12.3 | 8.8 | 1070 | 1070 | 8.2 | |||
| RCaMP1h | 571(594) | 575(602) | 65.1 | 18.7 | 0.51 | 0.14 | 97 | 7.7 | 10.0 | 10.9 | 1070 | 1070 | 8.1 | |||
| mApple | 568(592) | 568(592) | 57.3 | 0.42 | 70 | 1070 | 7.0 | |||||||||
| R-GECO1 | 564 (588) | 576(598) | 59.3 | 6.1 | 0.19 | 0.13 | 33 | 2.3 | 12.6 | 18.0 | 1065 | 1065 | 3.8 | |||