Fig. 1.
The IcmS–IcmW chaperon complex affects the translocation of effectors that received a low score by the HSMM. The WT strain JR32 (gray bars) and the icmS-icmW double-deletion mutant ED400 (diagonal striped bars) harboring the CyaA fusion proteins indicated below each bar were used to infect HL-60–derived human macrophages, and the cAMP levels of the infected cells were determined as described in Materials and Methods. The bar heights represent the means of the amount of cAMP per well obtained in at least three independent experiments; error bars indicate SDs. The cAMP levels of each fusion were found to be significantly different (*P < 0.05; **P < 0.01; ***P < 0.001, paired Student t test), comparing the WT strain and the icmS-icmW double-deletion mutant.