Figure 11.
Test for recognition of neuronal cells. A. Fetal-derived neural progenitor cells were transduced with a lentiviral vector expressing GFP or HLA-A*0201 and induced to differentiate into neural cells. Image of resultant GFP-transduced neural cells was as shown on the left. These cells were co-cultured with MAGE-A3 TCR transduced or untransduced (UnTd) T cells. As control for recognition, neural cell cultures were pulsed with MAGE-A3 peptide or as a negative control, SSX-2 peptide. B and C. Autologous dendritic cells were prepared from patient 5 and loaded with cell lysates from neuroblastoma cell line SK-N-SH, whole brain homogenate (BH), or pulsed with MAGE-A3 peptide (MAGE-A3). These were then co-cultured with T cells derived from patient 5; pre-treatment PBMC (periph), T cells derived from the ex vivo expanded CSF (CSF), the MAGE-A3 TCR transduced infusion cell product (TCR), or ex vivo cultured, untransduced T cells (UnTd). Reactivity was measured by CD107 amobilization (B) or 8-day cell proliferation measured by CFSE dilution (C). Reactivity in both CD8+ (left) and CD4+ (right) T cells was as shown.