FIGURE 2.
Incomplete dRTA in Rhcg+/− and Rhcg−/− mice. Mice were observed in metabolic cages under basal conditions and during an acid load for up to 7 days with HCl added to the diet (n = 5–10 per genotype). Rhcg−/− could only be observed for 6 days during the HCl-containing diet because most animals had to be terminated earlier. A, urinary ammonium excretion in 24-h urine collections normalized for urinary creatinine. B, urinary pH in 24-h urine collections. C, urinary net acid excretion (total bars) calculated from urinary ammonium (open bars) plus titratable acid (gray bars). Urinary bicarbonate levels were measured and negligible. D, blood bicarbonate concentrations. E, blood pH values. *, p < 0.05 for Rhcg−/− versus Rhcg+/+. ¥, p < 0.05 for Rhcg+/− versus Rhcg+/+.