Figure 9.
Comparison of the effects of PK treatment on pff-injected and uninjected aged PS19 mice. A, MC1 stained hippocampus (lateral CA3) and LC of K18/PL pff-injected mouse (top panels) and uninjected aged PS19 mouse (bottom panels) with no treatment or with 10 μg/ml PK treatment. Scale bar, 100 μm. B, Quantitative analyses of MC1-positive neurons in CA3 and LC. Data represents MC1 immunoreactivity normalized to no PK treatment conditions for both injected (n = 5) and uninjected aged (n = 4) PS19 mice. White bars represent no treatment, and black bars represent PK treatment. **p < 0.01, ***p < 0.001. C, Immunoblot analysis of cortex and hippocampus extracted sequentially with RIPA buffer, followed by 2% SDS. On each blot, the leftmost lane is uninjected age-matched control, and the other two lanes are two different injected mice (Injected) at 3 months after injection. mAb PHF1 detects hyperphosphorylated tau, and GAPDH was used as a loading control.