Figure 3. Knockdown of LRH-1 expression by siRNA downregulates CR-1 mRNA and protein expression in NTERA-2 cells.
Real time PCR for LRH-1 (A) and CR-1 (B) in NTERA-2 cells transfected with control or LRH-1 siRNAs. Data are representative of three experiments with duplicate samples. *P<0.05, as compared to control non-transfected samples. C: Western blot analysis for LRH-1, CR-1 and β-actin in siRNA transfected cells. D, E: Fold difference in LRH-1 and CR-1 expression by densitometric analysis of LRH-1 or CR-1 in NTERA-2 cell lysates normalized to β-actin content. *P<0.05, as compared to control non-transfected samples.
