Fig. 2.
NES activity of the C-terminal 16 amino acid residues of hAID. Confocal images of GFP signals with or without LMB (10 ng/ml for 150 min) are shown together with PI staining of the same field: GFP fusion proteins of JP8Bdel (A), 196X (B), JP8B-C9 (C), JP8B-C16 (D), C9 (F), C16 (E), and L172A (G). C16 and C9 indicate C-terminal peptides of 16 and 9 amino acid residues, respectively. The structure of each mutant is shown (Left). Arrowhead, point mutation; horizontal arrow, replacement; X, termination; black box, NES; crosshatched box, NLS.
