Figure 1. Effect of intrapulmonary K. pneumoniae challenge on expression of CRAMP.
(A) Wild type mice were challenged with i.t. K. pneumoniae. CRAMP expression at 0 hr, 6 hr, 24 hr, and 48 hr post-infection were assessed by real-time PCR and Western immunoblotting with densitometric analysis of whole lung homogenates (* p < 0.01, † p < 0.001 compared to control). Values are shown as mean ± SEM. mRNA and protein densitometry signify 4 mice/group, combined from two independent experiments. Western blotting is representative of 3 different mice/group. (B) AECs and pulmonary macrophages (Mac) were isolated from wild type mouse lungs. Cells were cultured in the presence of purified heat-killed K. pneumoniae (10:1 bacteria:cells). mRNA expression of CRAMP increases following exposure to bacteria in both cell types (* p < 0.01, † p < 0.001 compared to control time point). Values are expressed as mean ± SEM, and represent 3 wells/group, combined from 2 separate experiments.