Figure 2.
Fabp7 mRNA and protein enrichment cycles in the tripartite synapse. A, Analysis of mouse whole-brain mRNA by qPCR shows a dramatic change in the ratio of synaptoneurosomal (S) to total homogenate (H) Fabp7 mRNA compared with GAPDH and β-actin mRNA across the day. B, Analysis of mouse brain protein also shows a time-of-day cycling of Fabp7 enrichment in the ratio of synaptoneurosomal (S) to total homogenate (H). The cycling of the synaptic protein PSD-95 is also observed, but in a different phase to Fabp7. Light bars, light period; dark bars, dark period.