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. Author manuscript; available in PMC: 2013 Feb 5.
Published in final edited form as: Cell Rep. 2013 Jan 3;3(1):138–147. doi: 10.1016/j.celrep.2012.12.006

Figure 2. RPA interacts with resected ssDNA downstream of AID or I-PpoI endonuclease.

Figure 2

(A) RPA association with + and − DNA strands at the Grap gene locus from IgκAID-53BP1−/− activated B cells. RPKM values for the specified window (chr11:61,399,129-61,572,079) are provided in parenthesis. (B) RPA bound to Igh locus. As in (A), ChIP-Seq signals were resolved into upper and lower strands. Technical replicates were treated with E. coli ExoI or RecJ exonucleases nuclease prior to deep-sequencing library preparation. (C) PolII binding to + and − strands at the Grap locus. (D) Technical IP replicates from panels A and C samples incubated in the presence of ExoI. (E) Composite diagram showing RPA profiles at 19 I-PpoI mouse genomic sites in 53BP1−/− MEFs transduced with retroviruses expressing the I-PpoI homing endonuclease.