FIGURE 4.
C. pneumoniae degrades host TRAF3. (A) Whole cell lysates of uninfected and C. pneumoniae infected at 64 h p.i., or C. trachomatis infected at 24 h p.i. HeLa cells transfected with HA-TRAF3. Immunoblots were probed with anti-TRAF3 antibody. (B) C. pneumoniae cultures infected at an MOI of 1 or 0.5 and uninfected cells were transfected with HA-TRAF3. Resolved material was probed with anti-TRAF3 mAb. (C) Uninfected and chlamydiae infected HeLa cells transfected with Flag-TRAF2 were probed with anti-Flag mAb. Immunoblots probed with anti-actin were used as a loading control.