Figure 2. TSCM cells from healthy RMs are phenotypically and functionally distinct T cells.
(A) Expression of CXCR3 and CD122 on CD8+ TN, TSCM, TCM, and TEM cells by flow cytometry. (B) Percentage of CD8+ T cell subsets expressing CCR5, IL-18Rα, CD38, Ki-67, HLA-DR, or CXCR3. CD130 and CD122 are indicated as MFI. *P < 0.05 versus TSCM. (C) Pie charts representing the proportion of cells producing different combinations of IL-2, IFN-γ, and TNF after SEB stimulation. *P < 0.05, ***P < 0.001. (D) Mean percentage of cells with various differentiation phenotypes recovered following activation of FACS-sorted TN, TSCM, TCM, and TEM cells by αCD3/CD28 antibodies in vitro for 5 days (n = 6). The analysis was carried out on cells that diluted CFSE. Boxes in B and D show interquartile range and median values.