Figure 2.
Representative images of HpL cells incubated with (A) and without (B) 500 nM of fluorescently labeled Aβ42 that has undergone aggregation for 3–4 h. In each pair of images, the left is the phase contrast image, and the right is the fluorescent (TIRFM) image. Yellow species in the fluorescent image are oligomers (dually labeled), whereas green and red species are monomers (singly labeled). Scale bars are 3 μm. (C) Aβ40 and Aβ42 monomeric and oligomeric species were detectable over the limits of nonspecific interaction with the slide surface and over the background fluorescence of unlabeled cells 500 nM total peptide concentration, number of cells varied from 35 to 50 for each category, error bars are standard error of the mean, (SEM).