FIGURE 4.
BMI1 regulates DKK1 promoter and binds to it. A, the DKK1 promoter (1 kb upstream) was cloned in pGL4.18 luciferase reporter vector, and its activity was determined as described under “Experimental Procedures.” F and R represent Firefly and Renilla, respectively. Error bars represent ±S.D. *, p < 0.05 (Student's t test). B and C, binding of BMI1 and H3K27me3 to DKK1 promoter was determined using a ChIP assay in the indicated set of cells and four different sets of primers covering 1 kb of promoter region as described under “Experimental Procedures.” Ctrl, control.