Figure 4.
AMD3100 increases BM eNOS expression and the number of eNOS-expressing BM-derived cells in the AAR after IR injury. AMD3100 or saline was subcutaneously injected after IR injury in (A–D) WT mice and (E–H) WT mice that had been transplanted with BM from GFP-expressing mice. (A–C) BM was harvested from mice sacrificed before IR injury and from 1–14 days afterward. (A) BM cells were labeled with fluorescent anti-eNOS antibodies (red), and eNOS+ cells were quantified; scale bar=100 um. (B, C) BM plasma levels of (B) MMP-9 and (C) sKitL protein were determined via ELISA. (D) PB levels of nitrate and nitrite were determined via colorimetric assay before IR injury and 1–14 days afterward. (E) eNOS (red) and GFP (green) expression was evaluated in sections from the AAR of mice sacrificed 3 days after IR injury; scale bar=20 um. Cells positive for the expression of (F) eNOS, (G) GFP, or (H) both eNOS and GFP were quantified. #P<0.05 and ##P<0.01 versus before injection; *P<0.05 and **P<0.01 versus saline. Panels A–D: n=4–8 per treatment group at each time point; Panels F–H: n=3–5 per treatment group.