Fig. 6.
[PDL+STZ] enhances acinar-to-endocrine transdifferentiation. (A) [PDL+STZ] lineage-tracing schematic. (B-M) At [PDL+STZ] D30, no EYFP+ cells were present in duct or endocrine compartment in either (B) sham tail or (C) PDL head. EYFP+hormone+ cells were detected 30 days post-[PDL+STZ] in (D) the vicinity of ducts but (E) not in islets. At post-[PDL+STZ] D60, EYFP+ cells were only in acini in (F,J) sham tail and (G,K) PDL head, but numerous EYFP+endocrine-hormone+ cells in PDL tail were (H) apposed to ducts or (I) in islets. Of these, EYFP+insulin+ cells were found (L) near/in ducts or (M) in small islet clusters. (N,O) Quantitation of (N) total number EYFP+ endocrine cells and (O) total number EYFP+ islets (40 sections, ∼10% of PDL tail or head; n=3) at post-[PDL+STZ] D30 and D60; solid line indicates mean. S, sham tail; H, T: PDL head, tail. Scale bars: 50 μm.