. Author manuscript; available in PMC: 2014 Feb 1.

Published in final edited form as: J Cardiovasc Transl Res. 2012 Nov 15;6(1):22–30. doi: 10.1007/s12265-012-9423-2

Table 2.

Characterization of hiPSC-CM

Criteria	Assay(s)	Results
Electrophysiological properties	Multielectrode Array	Field potentials of beating cells including beat frequency, field potential duration (FPD), maximum positive amplitude (MPA), maximum negative amplitude (MNA), and interspike interval (ISI)
Electrophysiological properties	Patch Clamp	Action potential, classification of spontaneous action potentials (ventricular-, atrial-, or nodal-like)
Expression of cardiac-specific genes and proteins	Immunohistochemistry and/or RT-PCR	Positive for cardiac markers and proteins such as ACTN2, cTNT, Cx43, MLC2a, MYH6, TNNI3, and TNNT2
Structural properties and organization	Electron microscopy	Typical cardiomyocyte structural properties such as glycogen granules, mitochondria, myofibrils, sarcoplasmic reticulum striated sarcomeres, A-bands, I-bands, and Z-lines
Calcium signaling	Calcium imaging	Calcium transient waveforms, intracellular calcium voltage micromapping