FIGURE 6.
Analyses of the two suppressors of the nudAF208V mutant. A, both the nudAN356K and nudAT1133S mutations partially suppress the colony phenotype of the nudAF208V mutant. B, nuclear distribution phenotypes of the suppressors. Bar, 5 μm. C, a quantitative analysis of nuclear distribution in the suppressors. Percentage of cells in which the spore head contains four or more nuclei is presented. The mean and S.D. values are from data of three independent experiments (n > 200 DAPI-stained cells for each strain). The mean value of the nudAF208V,N356K or nudAF208V,T1133S mutant is lower than that of the nudAF208V mutant (p < 0.001). D, early endosome distribution phenotypes of the suppressors. E, a quantitative analysis on the frequency of dynein-mediated early endosome movements. Mean and S.D. values are shown (n = 35 hyphal tips for wild type, n = 45 for the nudAF208V mutant, n = 49 for the nudAF208V,N356K mutant, and n = 49 for the nudAF208V,T1133S mutant). The mean values of frequencies in the mutants within a time period of 16 s are relative to that of the wild type, which is set as 1. The mean value of the nudAF208V,N356K or nudAF208V,T1133S mutant is higher than that of the nudAF208V mutant (p < 0.001). F, a sequence alignment of dynein HCs showing the position of the suppressor mutation at Thr-1133. G, a sequence alignment of dynein HCs showing the position of the suppressor mutation at Asn-356.