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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: DNA Repair (Amst). 2012 Dec 12;12(2):121–127. doi: 10.1016/j.dnarep.2012.11.006

Figure 4.

Figure 4

Stable incorporation of ribonucleotides into DNA by proofreading proficient (Exo+) and deficient (Exo−) Leu606Met human Pol δ. (A) An alignment of motif A for human and yeast Pol δ is shown and the asterisk indicates the position of the Leu606Met mutation for human Pol δ. (B) Alkali cleavage products of reactions with all eight NTPs at cellular concentrations Leu606Met human Pol δ in the presence of PCNA. The relative amount of ribonucleotides incorporated into the primer strand is indicated below each lane. L indicates the ladder. (C) Average frequency of ribonucleotide incorporation by human Pol δ according to the incorporated ribonucleotide. (D) Frequency of ribonucleotide incorporation by human Pol δ at each of 24 template positions. Results are from at least two independent experiments.