FIGURE 8.
Perturbation of Fe-S biogenesis in crd1Δ. In the proposed model, loss of CL leads to decreased Fe-S biogenesis and maturation of Fe-S proteins, resulting in reduced activities of succinate dehydrogenase (II), ubiquinol-cytochrome c oxidoreductase (III), and aconitase (Aco1) in the mitochondria, as well as cytosolic Fe-S enzymes (Cyt Fe-S) sulfite reductase and isopropylmalate isomerase. Decreased Fe-S biogenesis is sensed by Aft1, which activates expression of the iron regulon genes, leading to increased mitochondrial iron levels.