Figure 7.
Localization of HXT1p in the periphery of fully developed haustoria and along the haustorial plasma membrane. (A) Superimposed Nomarski differential interference contrast and fluorescence images depicting two haustoria. Labeling of HXT1p with S651p resulted only in fluorescence signals in the periphery of the distal parts of the haustorium; proximal parts and haustorial neck are not labeled (visible on the left haustorium). h, haustorium; hn, haustorial neck. (Bar, 5 μm.) (B) Electron micrograph depicting considerable gold labeling along the haustorial plasma membrane (hpm) only (small arrows), but no labeling over the h, the extra haustorial matrix (ehma), the extrahaustorial membrane (ehm), or the plant cytoplasm (c). (Bar, 0.1 μm.)