FIGURE 2.
Tough Decoy inhibitors perform best among seven miRNA inhibition strategies when delivered by plasmid transfection or lentiviral transduction. A dual-luciferase assay was used to screen the potency of seven vector-encoded miRNA inhibitors targeting miR-16 (A,C) or miR-203 (B,D). miRNA inhibitor expression cassettes were delivered either by plasmid transfection (A,B) or by lentiviral transduction (C,D). A miR203-encoding plasmid was included in all assays evaluating miR-203 inhibitors. Data are depicted as mean + SEM. (*) P < 0.05, (**) P < 0.01, (***) P < 0.001, (****) P < 0.0001.