FIGURE 4.
Cellular localization of MTMR6 and Rab1B in NRK cells. A, immunostaining of NRK cells with anti-MTMR6 and anti-Rab1B antibodies. The arrowheads indicate MTMR6 and Rab1B concentrated at the cell center. The arrows indicate the peri-Golgi-localized MTMR6 and Rab1B. The insets show an enlarged view of the magnified boxed regions. It should be noted that the size and shape of the MTMR6 and Rab1B stainings are correlated in these three cells even if they are not completely overlapped. B, stable NRK cell line expressing EGFP-Rab1A was stained with anti-MTMR6 antibody. The arrowheads indicate EGFP-Rab1A and MTMR6 concentrated in the pericentrosomal region. C, NRK cells treated with DMSO or BFA (2 μg/ml) for 1 h at 37 °C were stained with anti-MTMR6 and anti-Rab1B antibodies or anti-β-COP antibody, respectively. Bars, 10 μm.