Table 1.
AntimiR oligonucleotides used in this study
| Oligonucleotide | Sequence (5′–3′) | Tm (°C) |
|---|---|---|
| antimiR-21 | GATAAGCT | 65 |
| antimiR-122 | CACACTCC | 78 |
| antimiR-155 | TAGCATTA | 61 |
| antimiR-221/222 | ATGTAGC | 56 |
| antilet-7 | ACTACCTC | 75 |
| 2′-O-Me antimiR-21 | gauaagcu | 37 |
| LNA mismatch | GGTAAACT | 39 |
| LNA scramble | TCATACTA | <30 |
LNA nucleotides are shown in uppercase and 2′-O-methyl–modified nucleotides are shown in lowercase. The LNA mismatch control had two mismatches (underlined) to the miR-21 seed region. We measured the Tm values of the LNA control oligonucleotides for the miR-21-LNA mismatch and the miR-21-LNA scramble duplexes.