Skip to main content
. Author manuscript; available in PMC: 2013 Nov 7.
Published in final edited form as: Cell Metab. 2012 Nov 7;16(5):579–587. doi: 10.1016/j.cmet.2012.10.003

Figure 2.

Figure 2

NTS L-leucine activates NTS S6K1 this signaling and this activation is required for NTS L-leucine’s anorexigenic effects.

(A) NTS S6K1 Thr389 phosphorylation (n=5) and (B) NTS pS6 immunoreactivity (n=4) in 24h fasted rats 30 min after a bilateral NTS aCSF or L-leucine injection. (C–E) Colocalization (right, orange) of DBH (C), TH (D) or POMC (E) (left, red) with pS6 (middle, green) in 24h fasted rats 30 min after a bilateral NTS L-leucine injection (n=4). (F) Food intake, first meal size and meal size following an icv injection of aCSF or SHU9119 combined with a bilateral injection of aCSF or L-leucine into the cmNTS. (G) Food intake, 24h body weight change, first meal size, mean meal size and cumulative meal number in rats following a bilateral injection of aCSF or L-leucine combined to a bilateral injection of DMSO or rapamycin into the cmNTS. Data are means ± SEM (n=12). *: p<0.05. scale bar: 100 μm.