Figure 2. Loss of Mob1a/1b promotes tumorigenesis.
(A and B) Tumor types arising in Mob1aΔ/Δ1btr/+ or Mob1aΔ/+1btr/tr mice. (A) Macroscopic or microscopic photographs of the indicated gross or H&E-stained tumors in mutant mice. Yellow arrows, tumor masses. Scale bars: 100 μm. (B) Kaplan-Meier analysis of tumor onset and survival for the Mob1aΔ/Δ1btr/+ (n = 31), Mob1aΔ/+1btr/tr (n = 48), and control Mob1aΔ/+1btr/+ (n = 22) mice in A. (C) Southern blots of tumor DNA showing loss of the WT Mob1 allele. Top (hybridized to probe A of Supplemental Figure 1A): lanes 1–3, control thymic DNA from Mob1a+/+, Mob1aΔ/+, and Mob1aΔ/Δ mice, respectively; lanes 4–5, osteosarcomas; 6–7, fibrosarcomas; 8–9, skin cancers; 10–11, breast cancers; 12–13, salivary gland cancers of Mob1aΔ/+1btr/tr mice. Bottom (probe C): 1–3, control tail DNA of Mob1b+/+, Mob1btr/+, and Mob1btr/tr mice, respectively; 4–5, osteosarcomas; 6–7, fibromyosarcomas; 8–9, skin cancers; 10–11, liver cancers of Mob1aΔ/Δ1btr/+ mice. Results shown are representative of at least 3 independent trials and at least 3 mice/group. Data are presented as the mean ± SEM, and P values were determined using the 2-tailed Student’s t test.