Fig. 3. Responses in the bitter hot spot are dependent on bitter taste receptor function.
(a) Different bitter compounds activate the same hot spot in the cortex (see Fig. S3) (n = 4). (b) Animals lacking the bitter taste receptor for cycloheximide (T2R5-KO) selectively lack cortical responses to cycloheximide, but retain normal responses to other bitters (e.g. 10 mM quinine) (n = 2, 7 trials each tastant). (c–d) Quantitation of taste responses in the control and T2R5-KO animals; also shown are data for a sweet tastant (AceK), note the lack of responses in both control and KO animals. Scale (a, b) = 100 µm, error bars are mean ± s.e.m.