Figure 2.
LV-induced IFN response in the liver is triggered by a TLR-independent pathway. The expression of OAS1, CXCL10, and ISG15 was determined by q-PCR in both the spleen and liver 4 hours after intravenous injection of LV.PGK.GFP (3 × 108 TU) in: (a) MyD88−/− mice (n = 3). (b) MyD88-TRIF−/− mice (n = 3). (c) RIG-I−/− mice (n = 3). All samples are presented as the fold-increase compared with the corresponding untreated controls. Results are the mean ± SD. *P < 0.05 versus LV-treated control mice. (d) AZT-treated mice. The retrotranscription inhibitor AZT was intraperitoneally injected (1 mg/kg) 20 minutes before an intravenous injection of LV (3 × 108 TU). Results are presented as the mean ± SD (n = 4). WT mice refers to littermate controls and were thus age, gender, and weight matched. Mice were not age or weight matched between the experiments done with different knockouts (i.e., between a, b, c, and d). In the case of the RIG-I knockout mice, WT mice are on a CD1/B6/129 background, whereas the MyD88 and MyD88TRIF knockout mice are on the C57Bl/6 background. *P < 0.05 versus LV-treated mice. AZT, azidothymidine; IFN, interferon; LV, lentiviral vector; q-PCR, quantitative-PCR; TLR, toll-like receptor; TU, transducing unit; unt, untreated; WT, wild-type.