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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Chromosoma. 2012 May 9;121(6):565–572. doi: 10.1007/s00412-012-0375-8

Figure 3. Plk1T210D is unable to phosphorylate BubR1 in vivo.

Figure 3

a Nocodazole-arrested cells were stained with pS676-BubR1 (green) and total BubR1 (red) antibodies (top panels) or total BubR1 (green) and Plk1 (red) antibodies (bottom panels). Scale bar represents 10 microns. b BubR1 and Plk1 signals were quantified from at least 50 kinetochores in 6 cells of each genotype. c Lysates from nocodazole-arrested cells were resolved by SDS-PAGE and immunoblotted with BubR1-specific antibodies.