Table II. Typical sets of parameters used in large scale parallel reaction monitoring analysis.
| Parameters | Multiplexed MS/MS analysis (parallel reaction monitoring) | ||||||
|---|---|---|---|---|---|---|---|
| Multiplexing degree | 1 | 1 | 2 | 4 | 4 | 8 | 8 |
| Isolation window/peptide (Th) | 2 | 2 | 2 | 2 | 2 | 2 | 1 |
| Max injection time/peptide (ms) | 1–60 | 1–120 | 1–30 | 1–30 | 1–30 | 1–30 | 1–15 |
| Transient length (ms) @ m/z = 200 Th | 64 | 128 | 64 | 128 | 128 | 256 | 128 |
| Resolution | 17.5k | 35k | 17.5k | 35k | 35k | 70k | 35k |
| Overall isolation window (Th) | 2 | 2 | 4 | 8 | 8 | 16 | 8 |
| Preset cycle time | 2 | 2 | 2 | 2 | 3 | 3 | 3 |
| Nb peptides analyzed/cycle time | 31 | 15 | 62 | 62 | 92 | 92 | 184 |