Figure 1.
Subpopulations of SPNs have dendrites in the cortical plate. A, Colocalization of two markers of SPNs. Immunohistochemistry for complexin 3 (red) in CTGF-GFP animals (green). Blue stain is DAPI to highlight cell bodies. Note that CTGF cells are in the same lamina as Cplx3 cells but not all CTGF cells express complexin 3 (arrowheads indicate two CTGF+ cells, one that did and one that did not costain with complexin3). Scale bar, 50 μm. The majority of GFP+ cells (>80%; n = 98 cells, n = 13 fields) are located throughout the upper half of the subplate. B, CTGF cells are not GABAergic. Immunohistochemistry for GABA (red) in CTGF-GFP animals (green) shows that CTGF cells do not contain GABA. Scale bar, 50 μm. C, CTGF cells extend dendrites into the cortical plate. Shown are apical neurites from two CTGF-expressing SPNs (left and right pairs of panels). The neurites of both cells are studded with spines, suggesting that they are dendrites. The boxed region in the left panel of each pair is shown at higher magnification in the corresponding right panel. Scale bars (in each pair of panels): 5 and 1 μm, respectively.