FIG. 12.
Induction of iNOS in mouse lung following RSV infection. (A) Balb/c mice were infected with RSV (1×107 pfu), and lung epithelial cell extracts were collected at various timepoints p.i. Proteins were resolved on an SDS–polyacrylamide gel and immunoblotted with a murine monoclonal antibody to iNOS. RSV infection produced an increase in iNOS protein compared to uninfected mice. (B) Nitrite production in BALF of BALB/C mice: Nitrite concentration was determined in BALF recovered at 24 h after RSV infection from regular mice and mice treated with CL2MBP to deplete macrophages. Nitrites were significantly increased by RSV alone, and significant reduction of nitrite production in AM depleted mice was observed. **p<0.001 AM depleted RSV-infected mice compared RSV-infected only. BALF, bronchoalveolar lavage fluid; CL2MBP, clodronate liposomes.