FIG. 1.
RSV replication and pathology. (A) Electron micrograph of RSV budding from an infected HEp-2 cell (Garofalo RP, personal observation). (B) IHC staining for RSV antigen of bronchiolar tissue from autopsy tissue of an infant with bronchiolitis. Brown staining indicates the presence of viral antigen in epithelial cells (reprinted by permission from Welliver et al.) (250). (C) IHC staining for RSV antigen in bronchoalveolar epithelial cells of the mouse lung. Mouse was inoculated with RSV (107 PFU), and lungs were collected at day 5 p.i. for staining (Garofalo RP, personal observation). (D) IHC staining of lung tissue for CD16 antigen (expressed primarily on neutrophils). Tissue was obtained from autopsy tissue of an infant with bronchiolitis (reprinted by permission from Welliver et al.) (250). (To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars.) IHC, immunohistochemical; p.i. postinfection; RSV, respiratory syncytial virus.