FIGURE 2.
H3K56ac is functionally involved in the oncogenic Ras signaling pathway. As detected using cell viability, soft agar, and Transwell assays, the presence of H3K56ac, which was mimicked by H3K56Q expression, reduced Ras-PI3K induced cell viability (A), colony formation (B), and cell migration (C). HeLa cells were transfected with pEGFP-N1, pEGFP-H3, pEGFP-H-RasG12V/Y40C, pEGFP-H3K56Q, or pEGFP-H3K18Q (indicated as GFP, H3, Ras, H3K56Q, and H3K18Q, respectively) plasmids. A, the H3, H3K18Q, or increasing amounts of the H3K56Q (0.5, 1, and 2 μg) expression plasmids were co-transfected with 0.6 μg of pEGFP-H-RasG12V/Y40C plasmid. pEGFP-H3K18Q was used as a control (2 μg). The numbers of colonies and the optical density (OD) values of the migrating cells are shown.